China Largest Factory Manufacturer Supply ≥98% Cholic Acid CAS 81-25-4 CAS NO.81-25-4

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Leader Biochemical Group is a large leader incorporated industry manufacturers and suppliers of advanced refined raw materials From the year of 1996 when our factory was put into production to year of 2020, our group has successively invested in more than 52 factories with shares and subordinates.We focus on manufacture Pharm & chemicals, functional active ingredients, nutritional Ingredients, health care products, cosmetics, pharmaceutical and refined feed, oil, natural plant ingredients industries to provide top quality of GMP standards products.All the invested factories' product lines cover API and intermediates, vitamins, amino acids, plant extracts, daily chemical products, cosmetics raw materials, nutrition and health care products, food additives, feed additives, essential oil products, fine chemical products and agricultural chemical raw materials And flavors and fragrances. Especially in the field of vitamins, amino acids, pharmaceutical raw materials and cosmetic raw materials, we have more than 20 years of production and sales experience. All products meet the requirements of high international export standards and have been recognized by customers all over the world. Our manufacture basement & R&D center located in National Aerospace Economic & Technical Development Zone Xi`an Shaanxi China. Now not only relying on self-cultivation and development as well as maintains good cooperative relations with many famous research institutes and universities in China. Now, we have closely cooperation with Shanghai Institute of Organic Chemistry of Chinese Academy of Science, Beijing Institute of Material Medical of Chinese Academy of Medical Science, China Pharmaceutical University, Zhejiang University. Closely cooperation with them not only integrating Science and technology resources, but also increasing the R&D speed and improving our R&D power. Offering Powerful Tech supporting Platform for group development. Keep serve the manufacture and the market as the R&D central task, focus on the technical research.  Now there are 3 technology R & D platforms including biological extract, microorganism fermentation and chemical synthesis, and can independently research and develop kinds of difficult APIs and pharmaceutical intermediates. With the strong support of China State Institute of Pharmaceutical Industry (hereinafter short for CSIPI), earlier known as Shanghai Institute of Pharmaceutical Industry (SIPI), we have unique advantages in the R & D and industrialization of high-grade, precision and advanced products.  Now our Group technical force is abundant, existing staff more that 1000 people, senior professional and technical staff accounted for more than 50% of the total number of employees, including 15 PhD research and development personnel, 5 master′ S degree in technical and management personnel 9 people. We have advanced equipment like fermentation equipment and technology also extraction, isolation, purification, synthesis with rich production experience and strict quality control system, According to the GMP required, quickly transforming the R&D results to industrial production in time, it is our advantages and our products are exported to North and South America, Europe, Middle East, Africa, and other five continents and scale the forefront in the nation, won good international reputation.  We believe only good quality can bring good cooperation, quality is our key spirit during our production, we are warmly welcome clients and partner from all over the world contact us for everlasting cooperation, Leader will be your strong, sincere and reliable partner in China.

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Cholic acid Basic information Chemical properties Uses Identification test Analysis of the content Toxicity Production methods Product Name: Cholic acid Synonyms: 3,7,12-Trihydroxy-cholan-24-oic acid;5beta-Cholan-24-oic acid, 3alpha,7alpha,12alpha-trihydroxy-;Cholan-24-oic acid, 3,7,12-trihydroxy-, (3alpha,5beta,7alpha,12alpha)-;3,7,12-TRIHYDROXYCHOLANIC ACID;3A,7A,12A-TRIHYDROXY-5B-CHOLAN-24-OIC ACID;3ALPHA, 7ALPHA, 12ALPHA-TRIHYDROXY-5BETA-CHOLANIC ACID;3ALPHA,7ALPHA,12ALPHA-TRIHYDROXY-5BETA-CHOLAN-24-IC ACID;3ALPHA,7ALPHA,12ALPHA-TRIHYDROXY-5BETA-CHOLAN-24-OIC ACID CAS: 81-25-4 MF: C24H40O5 MW: 408.58 EINECS: 201-337-8 Product Categories: Intermediates & Fine Chemicals;Pharmaceuticals;Steroids;chemical reagent;pharmaceutical intermediate;Inhibitors;phytochemical;reference standards from Chinese medicinal herbs (TCM).;standardized herbal extract Mol File: 81-25-4.mol   Cholic acid Chemical Properties Melting point  200-201 °C (lit.) alpha  36 º (c=0.6, 95% EtOH) Boiling point  449.08°C (rough estimate) density  1.0310 (rough estimate) refractive index  1.5800 (estimate) Fp  9℃ storage temp.  2-8°C solubility  methanol: 0.1 g/mL, clear form  neat pka 4.98(at 20℃) color  White to Brown Water Solubility  0.28 g/L (15 ºC) Merck  14,2203 BRN  2822009 InChIKey BHQCQFFYRZLCQQ-OELDTZBJSA-N CAS DataBase Reference 81-25-4(CAS DataBase Reference) NIST Chemistry Reference Cholic acid(81-25-4) EPA Substance Registry System Cholan-24-oic acid, 3,7,12-trihydroxy-, (3.alpha.,5.beta.,7.alpha.,12.alpha.)- (81-25-4)   Safety Information Hazard Codes  Xi Risk Statements  36/37/38 Safety Statements  24/25-36-26 RIDADR  UN1230 - class 3 - PG 2 - Methanol, solution WGK Germany  2 RTECS  FZ9350000 TSCA  Yes HS Code  29181990 Hazardous Substances Data 81-25-4(Hazardous Substances Data) MSDS Information Provider Language 3alpha,7alpha,12alpha-Trihydroxy-5beta-cholanic acid English ACROS English SigmaAldrich English ALFA English   Cholic acid Usage And Synthesis Chemical properties This agent exist in the bile cattle, sheep, pig. It is a colorless sheet or white crystalline powder. Some have a bitter to sweet taste. Its melting point is 198℃, specific rotation is (c = 0.6, ethanol) +37°. 1g cholic acid dissolved in about 300ml ethanol or acetone, 7ml glacial acetic acid. A small amount of cholic acid is soluble in water. The monohydrate was a white flake crystal. In 1927, H.Wieland (Germany) research accomplished bile acid composition, and won the Nobel Prize in chemistry. Uses (1) Emulsifiers. (2) Used for biochemical research, as pharmaceutical intermediates. Sodium cholate is a cholagogue, for the treatment of cholecystitis, bile deficiency, intestinal indigestion embolism. (3) As organic acids with steroid structure, cholic acid can emulsify fat, promote its digestion. (4) Non-denaturing ionic detergents for the extraction of membrane proteins. Identification test Solubility: hardly insoluble in water; soluble in ethanol. According to OT-42 method. The melting range : 197~202 ℃. As determined by conventional methods. Add 50% acetic acid solution to prepare 0.02% of the sample solution; take 1ml, 1% furfural solution, 6ml of water and concentrated sulfuric acid 5ml. This mixture should be converted into rosiness in 5min and then turn purple. Take about 10mg sample, add 2 drops of benzaldehyde and 3: 1 sulfuric acid 3 drops, heating at 50 ℃ for 5min. Plus glacial acetic acid about 10ml, then it should be brown. Analysis of the content Weigh about 400mg of the sample accurately; dried at 140 ℃ for 4h; put it into a 250ml flask; add 20ml of water and 40ml of ethanol; cover the surface dish, and gently heat to dissolve and cool in the steam bath. Add 5 drops of phenolphthalein test solution (TS-167), titrate with 0.1mol/L sodium hydroxide solution to pink and keep 15s, and make the necessary correction with the same time blank test. Each 1ml of 0.1 mol/L sodium hydroxide corresponds to 40.86 mg of cholic acid (C24 H40O5). Toxicity ADI O～1.25mg/kg(FAO/WH0，2001)。 ADI O~1.25 mg/kg (FAO/WH0, 2001)。 Production methods (1) Extracting from livestock (pigs, cattle, sheep, rabbits) the bile. 1.Ethanol crystallization method Preparation of crude cholic acid of cattle, sheep: take bovine or sheep bile; add 100 g/L sodium hydroxide; heat to boil for 12-18h to get saponification solution. Cooling. Adding acid to pH 1, precipitating cholic acid, removing bile acid, undergoing boiling and rinsing, drying at 75℃, milling can obtain bovine cholic acid. sodium cholate[NaOH] → [100 ℃, 12-18h] saponified solution[H2SO4] → [pH1, 75 ℃] crude cholic acid of cattle, sheep. Preparation of sodium cholate: add 0.5-1 times of crude cholic acid to 95% ethanol, and dissolve the solids by heating reflux method; cool. Broke the crystallization; filter; add 95% washing ethanol to make the filtrate colorless. Crystallize by adding 4 times the amount of ethanol; add 100-150g/L of activated carbon; dissolve the solids by heating reflux method; filter the liquid when it is hot. The filtrate concentrated to the original volume of 1/4. Through cooling, crystallization, filtration, adding ethanol to wash the crystallization can obtain the sodium cholate products. Crude cholic acid cattle, sheep [ethanol, activated carbon] → refined liquid [90 ℃ below] → sodium cholate products 2.  Ethyl acetate separation method Preparation of crude hyocholic acid: Add 3-3.5 times of saturated lime supernatant into fresh pig bile under stirring, then continue to stir it for 5-10min. heating to boiling for 2min, cooling, through filtration, adding hydrochloric acid to PH3.5 to get precipitation, standing for more than 12h can obtain crude acid. Removing, washing, adding 1.5 times the sodium hydroxide, plus 9 times the water, heating and boiling 12-18h, cooling, standing overnight obtain paste. Add water and sulfuric acid to pH 1 to precipitate pig cholic acid. Removing, crushing, rinsing to no acidity, through filtration gains crude pig cholic acid. [pig bile] [saturated limewater] →[100℃, pH 11-12] Basic filtrate [HCl] → [pH3.5] Crude cholic acid [water, NaOH] → paste [H2SO4] → [pH1] crude pig cholic acid Preparation of pig cholic acid products: add 4 times the amount of ethyl acetate to the crude pig cholic acid. Add 150-200g/L activated carbon; heat and flux for 0.5h; cool; filter; add 1.5-2.5 times ethyl acetate to filter cake; combine the filtrates twice. Adding 200g/L anhydrous sodium sulfate, standing overnight, concentrated to the original volume of 1/3, releasing, cooling crystallization, through filtration, washing with ethyl acetate crystallization, drying can obtain pig cholic acid products. Crude pig cholic acid [ethyl acetate, activated carbon] → filtrate [anhydrous sodium sulfate] → filtrate [concentration] → pig cholic acid products. Chemical Properties white to light beige crystalline powder Uses Choleretic produced by, and isolated from liver cells. Uses asthma therapeutic Uses Cholic Acid is an emulsifier that exists as colorless plates or a white crystalline powder which has a bitter taste with a sweetish aftertaste. it is slightly soluble in water. it functions as an emulsifying agent in egg white. Definition ChEBI: A bile acid that is 5beta-cholan-24-oic acid bearing three alpha-hydroxy substituents at position 3, 7 and 12. General Description This Certified Spiking Solution  is suitable as a starting material in preparation of linearity standards, calibrators, and controls for use in LC-MS/MS and GC/MS bile acid testing methods. Cholic acid is a primary bile acid that plays an important role in cholesterol homeostasis and intestinal absorption of dietary vitamins and lipids. Levels of cholic acid can serve as markers for inborn and acquired hepatobiliary disorders such as biliary atresia and familial intrahepatic cholestasis as well as inborn errors of bile acid synthesis and sclerosing cholangitis. Purification Methods This bile acid crystallises from H2O or wet Et2O (as hydrate) or EtOH (as alcoholate). Dry it under vacuum at 94o. When an alcoholic solution of cholic acid + I2 is added to aqueous KI, it forms a molecular compound (C24H40O5I)4 KI. H2O. The methyl ester is dimorphic with m 155o and 162o and [] D 20 +25o (EtOH). [Anderson et al. Biochem J 67 323 1957, 85 236 1962, Beilstein 10 III 2162, IV 2071.]   Cholic acid Preparation Products And Raw materials Raw materials Ethanol-->Ethyl acetate-->Sulfuric acid-->Taurine-->HYOCHOLIC ACID Preparation Products Atificial Cow-bezoar-->Glycocholic acid-->GLYCOCHOLIC ACID SODIUM SALT-->3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid-->Dehydrocholic acid-->Sodium taurocholate-->APOCHOLIC ACID