China Largest Manufacturer factory sales L-Histidine CAS 71-00-1 CAS NO.71-00-1

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L-Histidine Basic information Chemical properties Uses Production methods Content analysis Toxicity Using limits Product Name: L-Histidine Synonyms: (s)-1h-imidazole-4-alanin;(s)-1h-imidazole-4-propanoicaci;(s)-4-(2-amino-2-carboxyethyl)imidazole;(s)-alpha-amino-1h-imidazole-4-propanoicacid;1H-Imidazole-4-propanoic acid, alpha-amino-, (S)-;3-(1h-imidazol-4-yl)-l-alanin;4-(2-Amino-2-carboxyethyl)imidazole;L-HISTIDINE BASE CAS: 71-00-1 MF: C6H9N3O2 MW: 155.15 EINECS: 200-745-3 Product Categories: Amino Acids;Histidine [His, H];Amino Acids and Derivatives;alpha-Amino Acids;Biochemistry;Nutritional Supplements;Amino Acids;amino Mol File: 71-00-1.mol   L-Histidine Chemical Properties Melting point  282 °C (dec.)(lit.) alpha  12.4 º (c=11,6N HCl) Boiling point  278.95°C (rough estimate) density  1.3092 (rough estimate) FEMA  3694 | L-HISTIDINE refractive index  13 ° (C=11, 6mol/L HCl) storage temp.  2-8°C solubility  H2O: 50 mg/mL form  powder pka 1.8(at 25℃) color  White to off-white or pale yellow PH 7.0-8.0 (25℃, 0.1M in H2O) PH Range 7 - 8 at 15.5 g/l at 25 °C optical activity [α]20/D 39±1°, c = 2% in H2O Water Solubility  41.6 g/L (25 ºC) λmax λ: 260 nm Amax: 0.05 λ: 280 nm Amax: 0.05 JECFA Number 1431 Merck  14,4720 BRN  4673585 Stability: Stable. Incompatible with strong oxidizing agents. InChIKey HNDVDQJCIGZPNO-YFKPBYRVSA-N CAS DataBase Reference 71-00-1(CAS DataBase Reference) NIST Chemistry Reference L-Histidine(71-00-1) EPA Substance Registry System L-Histidine (71-00-1)   Safety Information Hazard Codes  Xn Risk Statements  22-36/37/38 Safety Statements  24/25-36/37-26-22 WGK Germany  2 RTECS  MS3070000 F  10-23 TSCA  Yes HS Code  29332990 Hazardous Substances Data 71-00-1(Hazardous Substances Data) Toxicity LD50 orally in Rabbit: > 5110 mg/kg MSDS Information Provider Language His English ACROS English SigmaAldrich English ALFA English   L-Histidine Usage And Synthesis Chemical properties White crystalline or crystalline powder,odorless,slightly bitter taste. Melting and decomposition at about 277~288 ℃. The imidazole and metal ions are easy to form complex salt. Dissolve in water (4.3g/100ml, 25 ℃), very difficult to dissolve in ethanol, insoluble in ether. It is commonly used for its hydrochloride, because of minimal solubility and other reasons. Uses (1) nutritional supplements. It is the very important components of Amino acid infusion and comprehensive amino acid preparations. It can be used in the treatment of gastric ulcer, anemia, allergies and so on. (2) It is used for biochemical research, medicine for the treatment of gastric ulcer, anemia, allergies and so on. (3) It is used as amino acid drugs. It is the main components of amino acid infusion and amino acid preparations, for the treatment of gastric ulcer, anemia and angina, aortitis, heart failure and other cardiovascular system disorders. Adverse reactions and contraindications: low toxicity, adult poisoning>64g/day, such as the injection of hydrochloric acid histidine with headache, flushing and heat. (4) It is used as a nutrient enhancer, the important component of amino acid infusion and amino acid preparations. It can be used for the treatment of gastric ulcer and biochemical researchment. (5) It is used for pharmaceutical raw materials and food additives. Production methods (1) It is eatracted from pig blood, bovine blood. Pig blood is spraied drying and then obtained blood powder, 100kg pig blood have 18kg blood power. L-histidine is commonly used as its hydrochloride salt ([7048-02-4]). The L-histidine containing eluent was concentrated to the appearance of crystals, adjusted to pH 2.5 with hydrochloric acid, and immediately added with 2 times the amount of ethanol in the solution, standing, precipitating and filtering to obtain L-group ammonia Acid hydrochloride crude, after decolorization, recrystallization, drying in the finished product. L-histidine can also be extracted from hydrolysates of defatted soybeans. There are two main production methods. First one is direct fermentation, with carbon source of glucose and an inducible drug-resistant strain of corynebacterium glutamicum. Second one is protein hydrolysis. The hydrolysis method is described in detail below. Pig and cattle blood , pig hair or hoof were raw materials, hydrolyzed by acid , separated and purificated to get L-histidine. Hydrolysis:50kg of pig blood powder and 4 times amout of 6mol/L HCl were put into a hydrolysis tank and heated at 110-120 ℃ for 24 hours. Preparation of the dilution of the column: The hydrolysis solution of the previous step was concentrated under reduced pressure, distilled water was added again, and the acid was repeatedly distilled for 3-4 times until the distillate did not flow out of the hydrochloric acid. Concentrated solution diluted with distilled water to 500L,adjusted pH to 3.5-4 with concentrated ammonia, plused 20% of the amount of blood powder activated carbon, heated, decolorizated and stirred at 90 ℃ for 6h.Then filtered when it is hot, and take the filtrate standing overnight precipitate. Filtering again, the filtrate was diluted with distilled water to 2.5% (according to the blood powder dosage), and adjusted to pH 2.5 with concentrated HCl, which was column dilution. The column dilution was separated, washed with water and eluted. Column is Ф300mm×2000 mm, PVC material, packed with 001 × 7 (732) strong acidic styrene cation exchange resin 1730mm, flow rate 1L/ min, stopping to upper column until the outflow of L histidine. Washing with water 500L, flow rate 1.5L /min. The pH 7.0-10.0 fraction was collected. After the collection, the resin was recoated for 15 min, and then regenerated with twice the amount of 1.5-2 mol/L HCl, the flow rate was 5-13 L/min. After regeneration, Washing with water untill PH 4 or so, waiting for the next column. Purification: L-histidine acid eluent, removing ammonia with vacuum, concentrated to dry, and then dissolved with 40L distilled water, adjusted pH with 3-3.26 mol/L HCl, add 1kg activated carbon, heated and decolorizated at 90 ℃ for 30min.The filtrate was concentrated in a thin film evaporator and allowed to stand for 48 hours to precipitate crystals. The crystals were collected by filtration, washed with 95% ethanol and dried at 80 oC with vacuum for 4 hours to give L-histidine hydrochloride. (2) Dry flour and hydrochloric acid as raw material reflux for several hours, filtrated,washed and handled with activated carbon to get L-histidine monohydrochloride crude, then purified to obtain the pure product. (3) separated with ion exchange resin from the protein hydrolyzate of the basic amino acid. Content analysis Sample is accurately weighed about 105 rag, dried at 105 ℃ for 3h, then dissolved in 3ml formic acid and 50ml glacial acetic acid, and titrated with 0.1mol/L perchloric acid, and the end point was determined by potentiometric method. At the same time a blank test and the necessary amendments should be made. Per Ml0.1mol/L perchloric acid equates to 15.52mg L-histidine (C6H9N3O2). Toxicity It is safe for using in food products (FDA § 172.320, 2000). Using limits Accounting for 2.4% of total protein in food (FDA § 172.320, 2000). FEMA: bakery products, meat products, dairy products, candy, frosting, are 150mg/kg. Description White, odorless crystals or crystalline powder having a slightly bitter taste. It is soluble in water, very slightly soluble in alcohol, and insoluble in ether. It melts with decomposition between about 277° and 288°C. Chemical Properties L-Histidine is an odorless powder with slightly bitter taste Chemical Properties White or almost white, crystalline powder or colourless crystals Occurrence Reported found in water bread, macaroni, egg noodles, corn flakes, corn grits, oatmeal, wheat bran, wheat flakes, shredded wheat, barley, brown rice, rye flour, whole grain wheat flour, buttermilk, blue cheese, cheddar cheese, cottage cheese, cream cheese, Parmesan cheese, bacon, cured ham, frankfurters, pork sausage, canned red kidney beans, canned sweet corn, canned peas, canned lima beans, canned potatoes, almonds, cashews, peanuts, dates, beef, lamb, veal, chicken, turkey and other natural sources Uses L-Histidine acts as a precursor to histamine and a component of carnosine. It is also used in medicine, feed additive, biochemical research, dietary supplement. It is used in a nutrition enhancer, is the amino acid fluid infusion. It can be used in the treatment of gastric ulcer. Uses L-Histidine is an essential amino acid for human development which the body cannot produce on its own. L-Histidine is one of the 22 proteinogenic amino acids. L-Histidine precursor to histamine (H4365 00) and a component of carnosine. Uses L-Histidine has been used for characterization of ZnO nanorod in an attempt to develop a technique for the ultra-low level detection of l-histidine. Definition ChEBI: The L-enantiomer of the amino acid histidine. Biotechnological Production L-Histidine is grouped with the aromatic amino acids, but the metabolic route diverges at an early stage from the other members of the group. It is produced in C. glutamicum in a 10-step sequence starting from phosphoribosyl pyrophosphate. Originally production titers of up to 10.5 g/L were reported, but this has since been increased by workers at Kyowa Hakko to 22.5 g/L. In a parallel development, the fermentation of L-histidine using E. coli has been reported by Ajinomoto, with titers up to 19.1 g/L. Both the titer and the carbon yield for L-histidine are lower than those reported for L-phenylalanine and L-tryptophan, and L-histidine remains one of the more challenging amino acids to produce on an industrial scale. Biochem/physiol Actions Precursor of histamine by action of histidine decarboxylase. Purification Methods A likely impurity is arginine. S-Histidine is adsorbed from aqueous solution onto a Dowex 50-H+ ion-exchange resin, washed with 1.5M HCl (to remove other amino acids), then eluted with 4M HCl as the dihydrochloride. This purified dihydrochloride (see below) is finally dissolved in water, the pH adjusted to 7.0, and the free zwitterionic base crystallises out on addition of EtOH. Its solubility in H2O is 4.2% at 25o. [Greenstein & Winitz The Chemistry of the Amino Acids J. Wiley, Vol 3 pp 1971-1993 1961, Beilstein 25 III/IV 4344.]   L-Histidine Preparation Products And Raw materials Raw materials D(+)-Glucose-->Α-PROTEIN-->L-Glutamic acid-->AMBERLITE(R) IRC-50-->Soy bean Isoflavone Isoflavone 10-40%-->Histamine-->2-MERCAPTO-L-HISTIDINE-->L-Carnosine Preparation Products L-Cystine-->Leuprorelin-->Bilirubin-->ANGIOTENSIN II, HUMAN-->Histamine-->N-Boc-L-Histidine-->Urocanic acid-->delta-guanidinovaleric acid-->SODIUM METHACRYLATE-->Hercynine

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Leader Biochemical Group is a large leader incorporated industry manufacturers and suppliers of advanced refined raw materials From the year of 1996 when our factory was put into production to year of 2020, our group has successively invested in more than 52 factories with shares and subordinates.We focus on manufacture Pharm & chemicals, functional active ingredients, nutritional Ingredients, health care products, cosmetics, pharmaceutical and refined feed, oil, natural plant ingredients industries to provide top quality of GMP standards products.All the invested factories' product lines cover API and intermediates, vitamins, amino acids, plant extracts, daily chemical products, cosmetics raw materials, nutrition and health care products, food additives, feed additives, essential oil products, fine chemical products and agricultural chemical raw materials And flavors and fragrances. Especially in the field of vitamins, amino acids, pharmaceutical raw materials and cosmetic raw materials, we have more than 20 years of production and sales experience. All products meet the requirements of high international export standards and have been recognized by customers all over the world. Our manufacture basement & R&D center located in National Aerospace Economic & Technical Development Zone Xi`an Shaanxi China. Now not only relying on self-cultivation and development as well as maintains good cooperative relations with many famous research institutes and universities in China. Now, we have closely cooperation with Shanghai Institute of Organic Chemistry of Chinese Academy of Science, Beijing Institute of Material Medical of Chinese Academy of Medical Science, China Pharmaceutical University, Zhejiang University. Closely cooperation with them not only integrating Science and technology resources, but also increasing the R&D speed and improving our R&D power. Offering Powerful Tech supporting Platform for group development. Keep serve the manufacture and the market as the R&D central task, focus on the technical research.  Now there are 3 technology R & D platforms including biological extract, microorganism fermentation and chemical synthesis, and can independently research and develop kinds of difficult APIs and pharmaceutical intermediates. With the strong support of China State Institute of Pharmaceutical Industry (hereinafter short for CSIPI), earlier known as Shanghai Institute of Pharmaceutical Industry (SIPI), we have unique advantages in the R & D and industrialization of high-grade, precision and advanced products.  Now our Group technical force is abundant, existing staff more that 1000 people, senior professional and technical staff accounted for more than 50% of the total number of employees, including 15 PhD research and development personnel, 5 master′ S degree in technical and management personnel 9 people. We have advanced equipment like fermentation equipment and technology also extraction, isolation, purification, synthesis with rich production experience and strict quality control system, According to the GMP required, quickly transforming the R&D results to industrial production in time, it is our advantages and our products are exported to North and South America, Europe, Middle East, Africa, and other five continents and scale the forefront in the nation, won good international reputation.  We believe only good quality can bring good cooperation, quality is our key spirit during our production, we are warmly welcome clients and partner from all over the world contact us for everlasting cooperation, Leader will be your strong, sincere and reliable partner in China.

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L-Histidine Basic information Chemical properties Uses Production methods Content analysis Toxicity Using limits Product Name: L-Histidine Synonyms: (s)-1h-imidazole-4-alanin;(s)-1h-imidazole-4-propanoicaci;(s)-4-(2-amino-2-carboxyethyl)imidazole;(s)-alpha-amino-1h-imidazole-4-propanoicacid;1H-Imidazole-4-propanoic acid, alpha-amino-, (S)-;3-(1h-imidazol-4-yl)-l-alanin;4-(2-Amino-2-carboxyethyl)imidazole;L-HISTIDINE BASE CAS: 71-00-1 MF: C6H9N3O2 MW: 155.15 EINECS: 200-745-3 Product Categories: Amino Acids;Histidine [His, H];Amino Acids and Derivatives;alpha-Amino Acids;Biochemistry;Nutritional Supplements;Amino Acids;amino Mol File: 71-00-1.mol   L-Histidine Chemical Properties Melting point  282 °C (dec.)(lit.) alpha  12.4 º (c=11,6N HCl) Boiling point  278.95°C (rough estimate) density  1.3092 (rough estimate) FEMA  3694 | L-HISTIDINE refractive index  13 ° (C=11, 6mol/L HCl) storage temp.  2-8°C solubility  H2O: 50 mg/mL form  powder pka 1.8(at 25℃) color  White to off-white or pale yellow PH 7.0-8.0 (25℃, 0.1M in H2O) PH Range 7 - 8 at 15.5 g/l at 25 °C optical activity [α]20/D 39±1°, c = 2% in H2O Water Solubility  41.6 g/L (25 ºC) λmax λ: 260 nm Amax: 0.05 λ: 280 nm Amax: 0.05 JECFA Number 1431 Merck  14,4720 BRN  4673585 Stability: Stable. Incompatible with strong oxidizing agents. InChIKey HNDVDQJCIGZPNO-YFKPBYRVSA-N CAS DataBase Reference 71-00-1(CAS DataBase Reference) NIST Chemistry Reference L-Histidine(71-00-1) EPA Substance Registry System L-Histidine (71-00-1)   Safety Information Hazard Codes  Xn Risk Statements  22-36/37/38 Safety Statements  24/25-36/37-26-22 WGK Germany  2 RTECS  MS3070000 F  10-23 TSCA  Yes HS Code  29332990 Hazardous Substances Data 71-00-1(Hazardous Substances Data) Toxicity LD50 orally in Rabbit: > 5110 mg/kg MSDS Information Provider Language His English ACROS English SigmaAldrich English ALFA English   L-Histidine Usage And Synthesis Chemical properties White crystalline or crystalline powder,odorless,slightly bitter taste. Melting and decomposition at about 277~288 ℃. The imidazole and metal ions are easy to form complex salt. Dissolve in water (4.3g/100ml, 25 ℃), very difficult to dissolve in ethanol, insoluble in ether. It is commonly used for its hydrochloride, because of minimal solubility and other reasons. Uses (1) nutritional supplements. It is the very important components of Amino acid infusion and comprehensive amino acid preparations. It can be used in the treatment of gastric ulcer, anemia, allergies and so on. (2) It is used for biochemical research, medicine for the treatment of gastric ulcer, anemia, allergies and so on. (3) It is used as amino acid drugs. It is the main components of amino acid infusion and amino acid preparations, for the treatment of gastric ulcer, anemia and angina, aortitis, heart failure and other cardiovascular system disorders. Adverse reactions and contraindications: low toxicity, adult poisoning>64g/day, such as the injection of hydrochloric acid histidine with headache, flushing and heat. (4) It is used as a nutrient enhancer, the important component of amino acid infusion and amino acid preparations. It can be used for the treatment of gastric ulcer and biochemical researchment. (5) It is used for pharmaceutical raw materials and food additives. Production methods (1) It is eatracted from pig blood, bovine blood. Pig blood is spraied drying and then obtained blood powder, 100kg pig blood have 18kg blood power. L-histidine is commonly used as its hydrochloride salt ([7048-02-4]). The L-histidine containing eluent was concentrated to the appearance of crystals, adjusted to pH 2.5 with hydrochloric acid, and immediately added with 2 times the amount of ethanol in the solution, standing, precipitating and filtering to obtain L-group ammonia Acid hydrochloride crude, after decolorization, recrystallization, drying in the finished product. L-histidine can also be extracted from hydrolysates of defatted soybeans. There are two main production methods. First one is direct fermentation, with carbon source of glucose and an inducible drug-resistant strain of corynebacterium glutamicum. Second one is protein hydrolysis. The hydrolysis method is described in detail below. Pig and cattle blood , pig hair or hoof were raw materials, hydrolyzed by acid , separated and purificated to get L-histidine. Hydrolysis:50kg of pig blood powder and 4 times amout of 6mol/L HCl were put into a hydrolysis tank and heated at 110-120 ℃ for 24 hours. Preparation of the dilution of the column: The hydrolysis solution of the previous step was concentrated under reduced pressure, distilled water was added again, and the acid was repeatedly distilled for 3-4 times until the distillate did not flow out of the hydrochloric acid. Concentrated solution diluted with distilled water to 500L,adjusted pH to 3.5-4 with concentrated ammonia, plused 20% of the amount of blood powder activated carbon, heated, decolorizated and stirred at 90 ℃ for 6h.Then filtered when it is hot, and take the filtrate standing overnight precipitate. Filtering again, the filtrate was diluted with distilled water to 2.5% (according to the blood powder dosage), and adjusted to pH 2.5 with concentrated HCl, which was column dilution. The column dilution was separated, washed with water and eluted. Column is Ф300mm×2000 mm, PVC material, packed with 001 × 7 (732) strong acidic styrene cation exchange resin 1730mm, flow rate 1L/ min, stopping to upper column until the outflow of L histidine. Washing with water 500L, flow rate 1.5L /min. The pH 7.0-10.0 fraction was collected. After the collection, the resin was recoated for 15 min, and then regenerated with twice the amount of 1.5-2 mol/L HCl, the flow rate was 5-13 L/min. After regeneration, Washing with water untill PH 4 or so, waiting for the next column. Purification: L-histidine acid eluent, removing ammonia with vacuum, concentrated to dry, and then dissolved with 40L distilled water, adjusted pH with 3-3.26 mol/L HCl, add 1kg activated carbon, heated and decolorizated at 90 ℃ for 30min.The filtrate was concentrated in a thin film evaporator and allowed to stand for 48 hours to precipitate crystals. The crystals were collected by filtration, washed with 95% ethanol and dried at 80 oC with vacuum for 4 hours to give L-histidine hydrochloride. (2) Dry flour and hydrochloric acid as raw material reflux for several hours, filtrated,washed and handled with activated carbon to get L-histidine monohydrochloride crude, then purified to obtain the pure product. (3) separated with ion exchange resin from the protein hydrolyzate of the basic amino acid. Content analysis Sample is accurately weighed about 105 rag, dried at 105 ℃ for 3h, then dissolved in 3ml formic acid and 50ml glacial acetic acid, and titrated with 0.1mol/L perchloric acid, and the end point was determined by potentiometric method. At the same time a blank test and the necessary amendments should be made. Per Ml0.1mol/L perchloric acid equates to 15.52mg L-histidine (C6H9N3O2). Toxicity It is safe for using in food products (FDA § 172.320, 2000). Using limits Accounting for 2.4% of total protein in food (FDA § 172.320, 2000). FEMA: bakery products, meat products, dairy products, candy, frosting, are 150mg/kg. Description White, odorless crystals or crystalline powder having a slightly bitter taste. It is soluble in water, very slightly soluble in alcohol, and insoluble in ether. It melts with decomposition between about 277° and 288°C. Chemical Properties L-Histidine is an odorless powder with slightly bitter taste Chemical Properties White or almost white, crystalline powder or colourless crystals Occurrence Reported found in water bread, macaroni, egg noodles, corn flakes, corn grits, oatmeal, wheat bran, wheat flakes, shredded wheat, barley, brown rice, rye flour, whole grain wheat flour, buttermilk, blue cheese, cheddar cheese, cottage cheese, cream cheese, Parmesan cheese, bacon, cured ham, frankfurters, pork sausage, canned red kidney beans, canned sweet corn, canned peas, canned lima beans, canned potatoes, almonds, cashews, peanuts, dates, beef, lamb, veal, chicken, turkey and other natural sources Uses L-Histidine acts as a precursor to histamine and a component of carnosine. It is also used in medicine, feed additive, biochemical research, dietary supplement. It is used in a nutrition enhancer, is the amino acid fluid infusion. It can be used in the treatment of gastric ulcer. Uses L-Histidine is an essential amino acid for human development which the body cannot produce on its own. L-Histidine is one of the 22 proteinogenic amino acids. L-Histidine precursor to histamine (H4365 00) and a component of carnosine. Uses L-Histidine has been used for characterization of ZnO nanorod in an attempt to develop a technique for the ultra-low level detection of l-histidine. Definition ChEBI: The L-enantiomer of the amino acid histidine. Biotechnological Production L-Histidine is grouped with the aromatic amino acids, but the metabolic route diverges at an early stage from the other members of the group. It is produced in C. glutamicum in a 10-step sequence starting from phosphoribosyl pyrophosphate. Originally production titers of up to 10.5 g/L were reported, but this has since been increased by workers at Kyowa Hakko to 22.5 g/L. In a parallel development, the fermentation of L-histidine using E. coli has been reported by Ajinomoto, with titers up to 19.1 g/L. Both the titer and the carbon yield for L-histidine are lower than those reported for L-phenylalanine and L-tryptophan, and L-histidine remains one of the more challenging amino acids to produce on an industrial scale. Biochem/physiol Actions Precursor of histamine by action of histidine decarboxylase. Purification Methods A likely impurity is arginine. S-Histidine is adsorbed from aqueous solution onto a Dowex 50-H+ ion-exchange resin, washed with 1.5M HCl (to remove other amino acids), then eluted with 4M HCl as the dihydrochloride. This purified dihydrochloride (see below) is finally dissolved in water, the pH adjusted to 7.0, and the free zwitterionic base crystallises out on addition of EtOH. Its solubility in H2O is 4.2% at 25o. [Greenstein & Winitz The Chemistry of the Amino Acids J. Wiley, Vol 3 pp 1971-1993 1961, Beilstein 25 III/IV 4344.]   L-Histidine Preparation Products And Raw materials Raw materials D(+)-Glucose-->Α-PROTEIN-->L-Glutamic acid-->AMBERLITE(R) IRC-50-->Soy bean Isoflavone Isoflavone 10-40%-->Histamine-->2-MERCAPTO-L-HISTIDINE-->L-Carnosine Preparation Products L-Cystine-->Leuprorelin-->Bilirubin-->ANGIOTENSIN II, HUMAN-->Histamine-->N-Boc-L-Histidine-->Urocanic acid-->delta-guanidinovaleric acid-->SODIUM METHACRYLATE-->Hercynine